Troubleshooting 2D Protein Gels in the Laboratory: A Comprehensive Guide
In protein analysis, 2D protein gels are an essential tool for separating complex mixtures of proteins based on both their isoelectric point (pI) and molecular weight. This powerful technique provides crucial insights into protein expression, modification, and structure, which is invaluable in pharmaceutical research, academic studies, and industrial applications. However, even experienced scientists face challenges…